Disruption of cells located in soil and sediments is attained one of two techniques. Just one procedure isolates the bacterial cells from the fabric just before the RNA isolation procedure. This can be achieved by homogenization of wet soil in a very Waring blender followed by a slow velocity centrifugation https://www.facebook.com/NewTechFind/posts/pfbid0d8VStcYignkfVz6MCZSKLaerVPpxVNKDb8CThGkvBTun4BMDH6o6PiDFY8YUrpQVl