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The cDNA sequence of human NAMPT was amplified by PCR from the pGex-6p-3-hNAMPT plasmid (kindly gifted from Dr Shui-qing YE, College of Missouri). The PCR goods were being digested and cloned into a pET21a+ vector utilizing NdeI and XhoI restriction enzymes. A degree mutation was launched by the fast change https://motherq000kwi4.bligblogging.com/profile


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